Molecular Phylogeny of Egyptian Sea Cucumbers As Predicted From 16s Mitochondrial rRNA Gene Sequences
نویسندگان
چکیده
Abstarct: A molecular phylogenetic analysis of some Holothuroidea was undertaken in order to clarify the systematics and taxonomy of this class in the red sea. DNA sequence for portions of 16S mitochondrial genes was obtained from 4 species of sea cucumbers. The resulting molecular phylogeny using maximum likelihood methods of the Holothuroidea conflicting existing taxonomy, which is based largely on the morphology of calcareous parts. In particular, evolutionary relationships among the brooding species were clarified. The current results confirmed that the species Holothuria fuscogilva, Actinopyga mauritiana, Holothuria nobilis and Sea cucumber leaves , were identical to Cucumaria frondosa, Pentacta pygmaea, and Cucumaria pseudocurata Psolus fabricii , respectively with Query coverage 100%, Zero Evalue and Max identical 100%. It was suggested that an in-depth phylogenetic analysis of sea cucumber families will require examination of additional, more slowly evolving, regions of the genome(s).
منابع مشابه
Phylogeny of Sea Cucumber (Echinodermata: Holothuroidea) as Inferred from 16S Mitochondrial rrna Gene Sequences (Filogeni Timun Laut (Echinodermata: Holothuroidea) Berdasarkan Jujukan Gen 16S Mitokondria rrna)
This study aimed to determine phylogenetic relationship between and among selected species of sea cucumbers (Echinodermata: Holothuroidea) using 16S mitochondrial ribosomal RNA (rRNA) gene. Phylogenetic analyses of 37 partial sequences of 16S mitochondrial rRNA gene using three main methods namely neighbour joining (NJ), maximum parsimony (MP) and maximum likelihood (ML) showed the presence of ...
متن کاملMitochondrial DNA sequence-based phylogenetic relationship of Trichiurus lepturus (Perciformes: Trichiuridae) from the Persian Gulf
In this study, mitochondrial DNA analysis using 16S ribosomal DNA (rDNA) was performed to investigate the phylogeny relationship of Trichiurus lepturus in the Persian Gulf compared to the other investigated area. The amplification of 16S rDNA resulted in a product of 600 bp in all samples. The results showed that the isolated strain belongs to T. lepturus showing 42 divergence sites among the s...
متن کاملA phylogeny analysis on six mullet species (Teleosti: Mugillidae) using PCR-sequencing method
In this study, genetic differences and phylogenic relationships among six Mugilidae species (Mugil cephalus, M. capito, Liza subviridis, L. saliens, L. aurata, Valamugil buchanani) were determined using PCR-sequencing. M. cephalus, L. subviridis, and V. buchanani from the Persian Gulf and Oman Sea, and L. aurata and L. saliens from the Caspian Sea were col-lected. Samples of an imported, Egypt...
متن کاملPhylogenetic Analysis of Beta-Glucanase Producing Actinomycetes Strain TBG-CH22 - A Comparison of Conventional and Molecular Morphometric Approach
Actinomycetes are inexhaustible producers of commercially valuable metabolites, are continually screened for beneficial compounds. The taxonomic and phylogenetic study of novel actinomycetes strains are mostly based on conventional methods and primary DNA structure of 16s rRNA. Although 16s rRNA sequence is well accepted in phylogeny studies, its secondary structures have not been widely used. ...
متن کاملPhylogenetic Analysis of Beta-Glucanase Producing Actinomycetes Strain TBG-CH22 - A Comparison of Conventional and Molecular Morphometric Approach
Actinomycetes are inexhaustible producers of commercially valuable metabolites, are continually screened for beneficial compounds. The taxonomic and phylogenetic study of novel actinomycetes strains are mostly based on conventional methods and primary DNA structure of 16s rRNA. Although 16s rRNA sequence is well accepted in phylogeny studies, its secondary structures have not been widely used. ...
متن کامل